Influence of Activators and Inhibitors on the Collagenase with Collagen Interaction Monitored by Dynamic Light Scattering in Solutions

Real–time digestion of type I collagen molecules by bacterial collagenase from Clostridium histolyticum has been monitored using Dynamic Light Scattering method. Time dependencies for translation diffusion coefficient (Dt) and hydrodynamic radius (RH) on were obtained pure “collagen + collagenase” Tris–HCl buffer solution at different temperatures solutions with added CaCl2, ZnCl2 MgCl2 EDTA. It was shown that is the first-order reaction. Reaction rate coefficients calculated.